Papers - NISHIYA Yoshiaki
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Development of a phenylpyruvate-specific dehydrogenase through structure-based enzyme designs and its application in a phenylpyruvate assay.
Nakamura, M. and Nishiya, Y.
International Journal of Analytical Bio-Science13 ( 2 ) 16 - 23 2025.06
Joint Work
Research paper (scientific journal)
Authorship: Corresponding author
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Chemical modification of glycine oxidase from Geobacillus kaustophilus by N-methylisothiazolinone.
Taneda, H., Higashiura, Y., Toyama F., and Nishiya, Y.
International Journal of Analytical Bio-Science13 ( 1 ) 10 - 15 2025.03
Joint Work
Research paper (scientific journal)
Authorship: Corresponding author
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Adaptive evolution of sesquiterpene deoxyphomenone in mycoparasitism by Hansfordia pulvinata associated with horizontal gene transfer from Aspergillus species.
Maeda, K., Sumita, T., Nishi, O., Sushida, H., Higashi, Y., Nakagawa, H., Suzuki, T., Iwao, E., Fanani, M. Z., Nishiya, Y., and Iida, Y.
mBio16 2025.03
Joint Work
Research paper (scientific journal)
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Rational alteration of the pH profile of sarcosine oxidase by site-directed mutagenesis around the active site.
Nishiya, Y., Ishihara, H., Zhang, Y., and Toyama, F.
International Journal of Analytical Bio-Science12 ( 3 ) 15 - 20 2024.09
Joint Work
Research paper (scientific journal)
Authorship: Lead author Corresponding author
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Chemical modification of Arthrobacter sarcosine oxidase by N-methylisothiazolinone reduces reactivity toward oxygen.
Toyama, F., Kimura, H., Zhang, Y., and Nishiya, Y.
Biosci. Biotechnol. Biochem.88 630 - 636 2024.06
Joint Work
Research paper (scientific journal)
Authorship: Corresponding author
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Development of a blocking peptide fragment from the hyperthermophilic bacterium (Thermotoga sp.).
Nishiya, Y., Takenawa, A., and Kiso, A.
International Journal of Analytical Bio-Science12 ( 2 ) 9 - 14 2024.06
Joint Work
Research paper (scientific journal)
Authorship: Lead author Corresponding author
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Computational insights for coenzyme interactions in wild-type and mutant sarcosine oxidases.
Nishiya, Y., Toyama, F., and Zhang, Y.
International Journal of Analytical Bio-Science11 ( 1 ) 111 - 116 2023.03
Joint Work
Research paper (scientific journal)
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信号累積型イオン感応性電界効果トランジスタによるアルカリホスファターゼ活性の簡易・低コスト検出.
佐々本康平，寺田理一郎，西矢芳昭
摂南大学融合科学研究所論文集8 ( 1 ) 31 - 43 2023.03
Joint Work
Research paper (scientific journal)
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Functional analysis of the N-terminal region of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis.
Sasamoto, K., Himiyama T., Moriyoshi, K., Ohmoto, T., Uegaki, K., Nakamura, T., and Nishiya, Y.
FEBS Open bio12 ( 10 ) 1875 - 1885 2022.10
Joint Work
Research paper (scientific journal)
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Reducing substrate inhibition of malate dehydrogenase from Geobacillus stearothermophilus by C-terminal truncation.
Shimozawa, Y., Matsuhisa, H., Nakamura T., Himiyama, T., and Nishiya, Y.
Protein Engineering Design and Selection35 2022.09
Joint Work
Research paper (scientific journal)
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Development of highly efficient variants of Pyroccocus Hippurate hydrolase for monitoring toluene and xylene exposure.
Yoshiaki Nishiya, Satsuki Imai, Shota Shinki, Kentaro Nagoshi, Toshiaki Baba
International Journal of Analytical Bio-Science10 ( 2 ) 42 - 47 2022.06
Joint Work
Research paper (scientific journal)
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Structural analysis of diagnostic enzymes : Differences in substrate specificity between malate dehydrogenase and lactate dehydrogenase.
下澤勇弥，氷見山幹基，中村努，西矢芳昭
生物試料分析45 151 - 159 2021.12
Joint Work
Research paper (scientific journal)
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Crystal structure of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis.
Sasamoto, K., Himiyama T., Moriyoshi, K., Ohmoto, T., Uegaki, K., Nishiya, Y., and Nakamura, T.
Acta Crystallographica77 399 - 406 2021.12
Joint Work
Research paper (scientific journal)
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Increasing loop flexibility affords low-temperature adaptation of a moderate thermophilic malate dehydrogenase from Geobacillus stearothermophilus.
Shimozawa, Y., Himiyama, T., Nakamura T., and Nishiya, Y.
Protein Engineering Design and Selection34 2021.11
Joint Work
Research paper (scientific journal)
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Protein engineering of D-succinylase from Cupriavidus sp. for D-amino acid synthesis and the structural implications.
Sumida, Y., Yamasaki, M., Nishiya, Y., Kumagai, S., Yamada, T., and Azuma, M.
Advanced Synthesis & Catalysis363 ( 20 ) 4770 - 4778 2021.10
Joint Work
Research paper (scientific journal)
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Analysis of a hippurate hydrolase homolog from Acetomicrobium mobile.
Yoshiaki Nishiya, Genta Torii, Muneyuki Suginaka, Satsuki Imai, Toshiaki Baba
International Journal of Analytical Bio-Science9 ( 3 ) 30 - 35 2021.09
Joint Work
Research paper (scientific journal)
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Structural analysis and reaction mechanism of malate dehydrogenase from Geobacillus stearothermophilus.
Yuya Shimozawa, Tomoki Himiyama, Tsutomu Nakamura, Yoshiaki Nishiya
Journal of Biochemistry170 ( 1 ) 97 - 105 2021.07
Joint Work
Research paper (scientific journal)
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Development of a hippuric acid-hydrolysing enzyme for monitoring toluene exposure.
Yoshiaki Nishiya, Kentaro Nagoshi, Shota Shinki, Satsuki Imai, Toshiaki Baba
International Journal of Analytical Bio-Science9 ( 2 ) 15 - 21 2021.06
Joint Work
Research paper (scientific journal)
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Structural prediction of 2-chloro-4-nitrophenyl-4-O-β-D-galactopyranosylmaltoside binding in the active site of human pancreatic α-amylase.
Yoshiaki Nishiya
生物試料分析44 ( 3 ) 83 - 88 2021.06
Single Work
Research paper (scientific journal)
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Simple phenol oxidase activity assay method based on a signal accumulation type of ion-sensitive field-effect transistor.
Yoshiaki Nishiya, Miyu Ueda, Yuka Yamashita, Naohiro Tomari, Yishihiro Yamamoto
International Journal of Analytical Bio-Science9 ( 1 ) 7 - 13 2021.03
Joint Work
Research paper (scientific journal)
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「相利」関係を目指した高大連携教育
佐々本 康平, 下澤 勇弥, 西矢 芳昭
工学教育研究講演会講演論文集68 2020.10
Joint Work
Research paper (scientific journal)
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Easy preparation of a stable membrane-bound lactate dehydrogenase for application on lactate biosensor.
Yuya Shimozawa, Saki Yoshida, Kazuya Ikeda, Yuri Kato, Fuka Toyama, Yoshiaki Nishiya
International Journal of Analytical Bio-Science8 ( 3 ) 65 - 70 2020.09
Joint Work
Research paper (scientific journal)
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Reaction mechanism of N-cyclopropylglycine oxidation by monomeric sarcosine oxidase.
Shoji, M., Abe, Y., Boero, M., Shigeta, Y., and Nishiya, Y.
Phys. Chem. Chem. Phys.22 16552 - 16561 2020.08
Joint Work
Research paper (scientific journal)
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Effect of aerobic, microaerobic, and anaerobic culture conditions on the morphology of Lactobacillus casei.
Yoshiaki Nishiya, Akane Nakamichi, Taichi Shimizu, Junpei Ohnishi, Shunsuke Aoki, Yuichi Oga
生物試料分析43 ( 3 ) 214 - 219 2020.06
Joint Work
Research paper (scientific journal)
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Structural prediction and analysis of the highly reactive alkaline phosphatase from Shewanella sp. T3-3.
Yuya Shimozawa, Hiroshi Aiba, Yoshiaki Nishiya
International Journal of Analytical Bio-Science8 ( 2 ) 39 - 43 2020.06
Joint Work
Research paper (scientific journal)
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発酵工学を基盤とした地域連携の試み.
西矢芳昭，居場嘉教，木村朋紀，船越英資
摂南大学地域総合研究所所報5 ( 1 ) 2020.03
Joint Work
Research paper (scientific journal)
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酵素特性を理解するための実験法の開発と高校生物教育への実践.
佐々本康平，下澤勇弥，北田広明，花本圭史，清水太一，松尾康光，西矢芳昭
摂南大学融合科学研究所論文集5 ( 1 ) 2019.12
Joint Work
Research paper (scientific journal)
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New enzymatic assays based on the combination of signal accumulation type of ion sensitive field effect transistor (SA-ISFET) with horseradish peroxidase.
Tomari, N., Sasamoto, K., Sakai, H., Tani, T., Yamamoto, Y., Nishiya, Y.
Anal. Biochem.584 113353 2019.11
Joint Work
Research paper (scientific journal)
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Altered substrate specificities of mandelate oxidase generated by site-directed mutagenesis of L-lactate oxidase.
Misaki Hiruta, Saki Yoshida, Yuya Shimozawa, Yoshiaki Nishiya
International Journal of Analytical Bio-Science7 ( 2 ) 35 - 39 2019.06
Joint Work
Research paper (scientific journal)
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検査薬の改良に役立つホモロジーモデリング: その限界の把握.
西矢芳昭
生物試料分析41 ( 5 ) 2018.12
Single Work
Research paper (scientific journal)
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Creation of an L-mandelate oxidase via structure-guided design of engineered lactate oxidase.
Hiruta, M. and Nishiya, Y.
Int. J. Anal. Bio-Sci.6 ( 2 ) 25 - 29 2018.06
Joint Work
Research paper (scientific journal)
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酵素立体構造および反応予測に基づく尿素窒素のダブルカイネティックアッセイの考察.
西矢芳昭
生物試料分析41 ( 3 ) 162 - 167 2018.06
Single Work
Research paper (scientific journal)
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Characterization of D-succinylase from Cupriavidus sp. P4-10-C and its application in D-amino acid synthesis.
Sumida, Y., Iwai, S., Nishiya, Y., Kumagai, S., Yamada, T., Azuma, M.
Journal of Bioscience and Bioengineering125 ( 3 ) 282 - 286 2018.03
Joint Work
Research paper (scientific journal)
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信号累積型イオン感応性電界効果トランジスタの応用.
佐々本康平，西矢芳昭
有機・バイオイオニクス研究会講演概要論文集2 5 - 6 2018.03
Joint Work
Research paper (conference, symposium, etc.)
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酵素によるプロトンソースの生成とバイオセンサーへの応用.
瀬溝人生，西矢芳昭，松尾康光
有機・バイオイオニクス研究会講演概要論文集2 21 - 22 2018.03
Joint Work
Research paper (conference, symposium, etc.)
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シミュレーションに基づく臨床検査用酵素の開発.
下澤勇弥，西矢芳昭
有機・バイオイオニクス研究会講演概要論文集2 19 - 20 2018.03
Joint Work
Research paper (conference, symposium, etc.)
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Over-expression, characterization and modification of highly active alkaline phosphatase from a Shewanella genus bacterium.
Aiba, H., Nishiya, Y., Azuma, M.
Biosci. Biotechnol. Biochem.81 1994 - 2001 2017.07
Joint Work
Research paper (scientific journal)
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The reaction mechanism of sarcosine oxidase elucidated using FMO and QM/MM methods.
Abe Y, Shoji M, Nishiya Y, Aiba H, Kishimoto T, Kitaura K
Phys. Chem. Chem. Phys.19 9811 - 9822 2017.05
Joint Work
Research paper (scientific journal)
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Structural prediction and interpretation of the substrate specificity of a lactate oxidase from Enterococcus sp. NBRC3427.
Misaki Hiruta, Yoshiaki Nishiya
Int. J. Anal. Bio-Sci.5 ( 2 ) 30 - 36 2017.04
Joint Work
Research paper (scientific journal)
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バイオセンサ用酵素としての乳酸オキシダーゼ.
西矢芳昭，比留田美咲
有機・バイオイオニクス研究会講演概要論文集1 11 - 13 2017.03
Joint Work
Research paper (conference, symposium, etc.)
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Geobacillus stearothermophilus由来Malate dehydrogenaseの特性評価と機能改変.
下澤勇弥，西矢芳昭
有機・バイオイオニクス研究会講演概要論文集1 21 - 22 2017.03
Joint Work
Research paper (conference, symposium, etc.)
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L-Thioproline: a new substrate for monomeric sarcosine oxidase selected by in silico analysis.
Yoshiaki Nishiya, Chisa Yoshida, Tasuku Okajima
International Journal of Analytical Bio-Science4 ( 4 ) 83 - 91 2016.12
Joint Work
Research paper (scientific journal)
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生物教育実験のための市販キノコ・ダイレクトPCR法
青木駿介，西矢芳昭
摂南大学融合科学研究所論文集2 10 - 17 2016.10
Joint Work
Research paper (bulletin of university, research institution)
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Monomeric sarcosine oxidase exhibiting high substrate affinity and thermostability.
Yoshiaki Nishiya, Machi Yamamoto, Jun-ichi Takemoto, Shusaku Kano, Shohei Nakano
International Journal of Analytical Bio-Science4 ( 3 ) 55 - 62 2016.09
Joint Work
Research paper (scientific journal)
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Properties of Geobacillus stearothermophilus malate dehydrogenase used as a diagnostic reagent and its characterization by molecular modeling.
Yoshiaki Nishiya, Yuya Shimozawa
International Journal of Analytical Bio-Science4 ( 2 ) 21 - 27 2016.06
Joint Work
Research paper (scientific journal)
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Identification and characterization of D-succinylase, and a proposed enzymatic method for D-amino acid synthesis.
Yosuke Sumida, Sachio Iwai, Yoshiaki Nishiya, Shinya Kumagai, Toshihide Yamada, Masayuki Azuma
Advanced Synthesis & Catalysis358 2041 - 2046 2016.06
Joint Work
Research paper (scientific journal)
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Hyperstabilization of Tetrameric Bacillus sp. TB-90 Urate Oxidase by Introducing Disulfide Bonds through Structural Plasticity.
Takao Hibi, Asami Kume, Akie Kawamura, Takafumi Itoh, Harumi Fukada, and Yoshiaki Nishiya
Biochemistry55 ( 4 ) 724 - 732 2016.01
Joint Work
Research paper (scientific journal)
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Comparison of the substrate specificity of L-pipecolate oxidase and bacterial monomeric sarcosine oxidase, and structural interpretation of the enzymes.
Yoshiaki Nishiya and Yukihiro Abe
Int. J. Anal. Bio-Sci.3 ( 3 ) 55 - 62 2015.09
Joint Work
Research paper (scientific journal)
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Development of a bioactivity analyzer using a signal accumulation type of ion-sensitive field-effect transistor
Toshio Tani and Yoshiaki Nishiya
生物試料分析38 ( 3 ) 196 - 201 2015.06
Joint Work
Research paper (scientific journal)
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Characterization of a thermostable glucose dehydrogenase with strict substrate specificity from a hyperthermophilic archaeon Thermoproteus sp. GDH-1.
Hiroshi Aiba, Yoshiaki Nishiya, Masayuki Azuma, Yuusuke Yokooji, Haruyuki Atomi & Tadayuki Imanaka
Biosci. Biotechnol. Biochem.79 ( 7 ) 1094 - 1102 2015.05
Joint Work
Research paper (scientific journal)
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A simple and reliable urea assay method based on a signal accumulation type of ion-sensitive field-effect transistor.
Tomari, N., Kawasaki, A., Yamamoto, Y., and Nishiya, Y.
J. Biosci. Bioeng.119 247 - 250 2015.02
Joint Work
Research paper (scientific journal)
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Engineering SS bridge of thermostable mutant of Bacillus sp. TB-90 urate oxidase.
Hibi, T., Hayashi, Y., Itoh, T., and Nishiya, Y.
Photon Factory Activity Report 2015
Joint Work
Research paper (bulletin of university, research institution)
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Crystal structure of glycine oxidase from Geobacillus kaustophilus.
Shiono, T., Nomura, T., Nishiya, Y, and Arai, R.
Photon Factory Activity Report 2015
Joint Work
Research paper (bulletin of university, research institution)
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Structural comparison of creatinases for investigating substrate binding.
Yoshiaki Nishiya
Int. J. Anal. Bio-Sci.2 ( 4 ) 143 - 147 2014.12
Single Work
Research paper (scientific journal)
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Intersubunit salt bridges with a sulfate anion control subunit dissociation and thermal stabilization of Bacillus sp. TB-90 urate oxidase.
Hibi, T., Hayashi, Y., Fukada, H., Itoh, T., Nago, T., and Nishiya, Y.
Biochemistry53 3879 - 3888 2014.07
Joint Work
Research paper (scientific journal)
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Screening of enzyme stabilizers using thermal shift assays on the basis of structural informations.
Nishiya, Y. and Nakano, S.
Int. J. Anal. Bio-Sci.2 ( 2 ) 58 - 63 2014.06
Joint Work
Research paper (scientific journal)
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Intersubunit salt bridges of Bacillus sp. TB-90 urate oxidase.
Hibi, T., Hayashi, Y., Itoh, T., and Nishiya, Y.
Photon Factory Activity Report 2014
Joint Work
Research paper (bulletin of university, research institution)
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Homology modeling and docking study of creatinine deiminase.
Yoshiaki Nishiya
Int J Anal Bio-Sci1 ( 2 ) 55 - 59 2013.12
Single Work
Research paper (scientific journal)
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Altered substrate affinity of monomeric sarcosine oxidase by the mutation of phenylalanine-103 or histidine-348.
Yoshiaki Nishiya
Int. J. Anal. Bio-Sci.1 ( 1 ) 21 - 26 2013.09
Single Work
Research paper (scientific journal)
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好冷化変異体ウリカーゼの予備的X線結晶構造解析.
日び隆雄，伊藤貴文，西矢芳昭
Photon Factory Activity Report 2012
Joint Work
Research paper (bulletin of university, research institution)
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Monomeric sarcosine oxidase acts on both L- and D-substrates.
Nishiya, Y., Nakano, S., Kawamura, K, and Abe, Y.
J. Anal. Bio-Sci.35 426 - 430 2012
Joint Work
Research paper (scientific journal)
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Structure-guided mutagenesis for the improvement of substrate specificity of Bacillus megaterium glucose 1-dehydrogenase IV.
Nishioka, T., Yasutake, Y., Nishiya, Y., and Tamura, T.
FEBS J.279 3264 - 3275 2012
Joint Work
Research paper (scientific journal)
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Structural and mutational analyses of Bacillus megaterium glucose 1-dehydrogenase IV.
Nishioka, T., Yasutake, Y, Nishiya, Y., and Tamura, T.
Photon Factory Activity Report 2012
Joint Work
Research paper (bulletin of university, research institution)
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信号累積型イオン感受性電界効果トランジスタによるコレステロールエステルの新規測定法.
西矢芳昭，廣岡青央，谷敏夫，泊直宏，高坂千尋，山本佳宏
生物試料分析34 247 - 250 2011
Joint Work
Research paper (scientific journal)
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IS-excision enhancer removes transposable elements from bacterial genomes and induces various genomic deletions.
Kusumoto, M., Oota, T., Nishiya, Y., Ogura, Y., Saito, T., Sekine, Y., Iwata, T., Akiba, M., and Hayashi, T.
Nat. Commun.2 152 2011
Joint Work
Research paper (scientific journal)
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信号累積型のイオン感応性電界効果トランジスタによる腎臓疾患マーカーおよびトランスポーターの測定法.
西矢芳昭，谷敏夫
BioClinica26 62 - 67 2011
Joint Work
Research paper (scientific journal)
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Mutational and crystallographic analyses of Thermoplasma acidophilum D-aldohexose dehydrogenase C-terminal deletion mutants.
Nishioka, T., Yasutake, Y., Nishiya, Y., Tamura, N., and Tamura, T.
Photon Factory Activity Report 2010
Joint Work
Research paper (bulletin of university, research institution)
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Alteration of L-proline oxidase activity of sarcosine oxidase and a structural interpretation.
Nishiya, Y. and Kishimoto, T.
J. Anal. Bio-Sci.33 161 - 166 2010
Joint Work
Research paper (scientific journal)
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Improvement of thermal stability of Leuconostoc psudomesenteroides glucose-6-phosphate dehydrogenase.
Kusumoto, M., Kishimoto, T., and Nishiya, Y.
J. Anal. Bio-Sci.33 397 - 400 2010
Joint Work
Research paper (scientific journal)
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Substitution of Glu122 by glutamine revealed the function of the second water molecule as a proton donor in the binuclear metal enzyme creatininase.
Yamashita, K., Nakajima, Y., Matsushita, H., Nishiya, Y., Yamazawa, R., Wu, Y. F., Matsubara, F., Oyama, H., Ito, K., and Yoshimoto, T.
J. Mol. Biol.396 1081 - 1096 2010
Joint Work
Research paper (scientific journal)
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信号累積型イオン感受性電界効果トランジスタによるクレアチニンの新規測定法.
西矢芳昭，谷敏夫，廣岡青央，泊直宏，高坂千尋，山本佳宏
生物試料分析32 240 - 243 2009
Joint Work
Research paper (scientific journal)
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Improvement of thermal stability of Saccharomyces pastorianus hexokinase by random mutagenesis and a structural interpretation.
Nishiya, Y. and Kishimoto, T.
J. Anal. Bio-Sci.32 307 - 312 2009
Joint Work
Research paper (scientific journal)
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C-terminal tail derived from neighboring subunit is critical for the activity of Thermoplasma acidophilum D-aldohexose dehydrogenase.
Nishioka, T., Yasutake, Y., Nishiya, Y., Tamura, N., and Tamura, T.
Proteins74 801 - 807 2009
Joint Work
Research paper (scientific journal)
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バイオ用途への応用を目的とした磁性アミロースビーズの開発.
岸本幹雄，坪井聡子，臼杵直樹，河野研二，西矢芳昭，楠本正博
粉体および粉末冶金54 671 - 674 2008
Joint Work
Research paper (scientific journal)
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Stabilization mechanism of chloride ion on thermal denaturation of Arthrobacter sarcosine oxidase.
Sugimoto, H., Nishiya, Y., Miyake, H., and Tanaka, A.
Netsu Sokutei35 76 - 80 2008
Joint Work
Research paper (scientific journal)
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分析用酵素の工業生産における宿主菌の活用
西矢芳昭，山本和巳，竹嶋誠嗣
酵素工学ニュース ( 58 ) 44 - 49 2007.10
Joint Work
Research paper (scientific journal)
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Characterization of a NADH:dichloroindophenol oxidoreductase from Bacillus subtilis.
Nishiya, Y., and Yamamoto, Y.
Bioscience.Biotechnology and Biochemistry71 ( 2 ) 611 - 614 2007.02
Joint Work
Research paper (scientific journal)
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Structural insights into unique substrate selectivity of Thermoplasma acidophilum D-aldohexose dehydrogenase.
Yasutake, Y., Nishiya, Y., Tamura, N., and Tamura, T.
J. Mol. Biol.367 1034 - 1046 2007
Joint Work
Research paper (scientific journal)
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Crystal structures of Thermoplasma acidophilum D-aldohexose dehydrogenase.
Yasutake, Y., Nishiya, Y., Tamura, N., and Tamura, T.
Photon Factory Activity Report 2007
Joint Work
Research paper (bulletin of university, research institution)
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ヒドロキシアパタイトコーティング磁性ナノ粒子の開発と応用.
西矢芳昭，臼杵直樹，楠本正博，河野研二，岸本幹雄
生物試料分析29 429 - 434 2006
Joint Work
Research paper (scientific journal)
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Characterization of the creatininase from Alcaligenes faecalis.
Nishiya, Y. and Toda, A.
J. Anal. Bio-Sci.29 249 - 253 2006
Joint Work
Research paper (scientific journal)
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Preliminary crystallographic studies of the aldohexose dehydrogenase from Thermoplasma acidophilum.
Yasutake, Y., Nishiya, Y., Tamura, N., and Tamura, T.
Acta Cryst.F62 586 - 589 2006
Joint Work
Research paper (scientific journal)
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Homogeneous enzymatic assay for L-cysteine with C-S lyase.
Nishiya, Y., Yoshida, Y., Yoshimura, M., Fukamachi, H., and Nakano, Y.
Bioscience.Biotechnology and Biochemistry69 ( 11 ) 2244 - 2246 2005.11
Joint Work
Research paper (scientific journal)
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Detection and quantification of on-chip phosphorylated peptides by surface plasmon resonance imaging techniques using a phosphate capture molecule.
Inamori, K., Kyo, M., Nishiya, Y., Inoue, Y., Sonoda, T., Kinoshita, E., Koike, T., and Katayama, Y.
Anal. Chem.77 3979 - 3985 2005
Joint Work
Research paper (scientific journal)
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Analysis of bacterial glucose dehydrogenase homologs from thermoacidophilic archaeon Thermoplasma acidophilum: finding and characterization of aldohexose dehydrogenase.
Nishiya, Y., Tamura, N., and Tamura, T.
Bioscience.Biotechnology and Biochemistry68 ( 12 ) 2451 - 2456 2004.12
Joint Work
Research paper (scientific journal)
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耐熱性アルドヘキソースデヒドロゲナーゼの発見とＤ－マンノース測定への応用
西矢芳昭，田村範子，田村具博
検査と技術32 679 - 681 2004.07
Joint Work
Research paper (scientific journal)
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二核亜鉛錯体「フォスタグ」によるNADP依存性酵素活性の阻害とリン酸イオン濃度依存的な再活性化.
西矢芳昭，辻勝巳，小池透
生物試料分析27 244 - 248 2004
Joint Work
Research paper (scientific journal)
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Host-dependent activation of IS1203v excision in Shiga toxin-producing Escherichia coli.
Kusumoto, M., Suzuki, R., Nishiya Y., Okitsu, T., and Oka, M.
J. Biosci. Bioeng.97 406 - 411 2004
Joint Work
Research paper (scientific journal)
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バイオ実験ロボットの開発
小嶋浩樹，竹本誠，池田勝徳，西矢芳昭
バイオインダストリー20 5 - 12 2003.02
Joint Work
Research paper (scientific journal)
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高泡性を示す清酒酵母に特異的に発現するタンパク質.
山本佳宏，廣岡青央，西矢芳昭，筒井延男
日本生物工学会誌81 461 - 467 2003
Single Work
Research paper (scientific journal)
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診断用酵素サルコシンオキシダーゼにおける構造と機能の関連性.
西矢芳昭，平山令明
生物試料分析26 191 - 195 2003
Joint Work
Research paper (scientific journal)
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Gene cloning and polymerase chain reaction with replication factor C from Thermococcus kodakaraensis KOD1.
Kitabayashi, M., Nishiya, Y., Esaka, M., Itakura, M., and Imanaka, T.
Biosci. Biotechnol. Biochem.67 2373 - 2380 2003
Joint Work
Research paper (scientific journal)
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A simple and efficient method for high fidelity PCR cloning using antibody-neutralizing technology.
Kitabayashi, M., Nishiya, Y., Esaka, M.
Biosci. Biotechnol. Biochem.67 2034 - 2037 2003
Joint Work
Research paper (scientific journal)
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A purification method of the diagnostic enzyme Bacillus uricase using magnetic beads and non-specific protease.
Nishiya, Y., Hibi, T., and Oda, J.
Protein Expr. Purif.25 426 - 429 2002
Joint Work
Research paper (scientific journal)
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磁気分離技術を利用したHis-tagタンパク質の精製システム.
西矢芳昭，北林雅夫，池田勝徳
実験医学20 479 - 482 2002
Joint Work
Research paper (scientific journal)
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Gene cloning and polymerase chain reaction with proliferating cell nuclear antigen from Thermococcus kodakaraensis KOD1.
Kitabayashi, M., Nishiya, Y., Esaka, M., Itakura, M., and Imanaka, T.
Biosci. Biotechnol. Biochem.66 2194 - 2200 2002
Joint Work
Research paper (scientific journal)
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Preliminary crystallographic studies of the creatinine amidohydrolase from Pseudomonas putida.
Ito, K., Kanada, N., Inoue, T., Furukawa, K., Yamashita, K., Tanaka, N., Nakamura, KT., Nishiya, Y., Sogabe, A., and Yoshimoto, T.
Acta Crystallogr. D Biol. Crystallogr.58 2180 - 2181 2002
Joint Work
Research paper (scientific journal)
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Biochemical and molecular analysis of the Arthrobacter creatinase.
Nishiya, Y. and Toda, A.
J. Anal. Bio-Sci.25 392 - 398 2002
Joint Work
Research paper (scientific journal)
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Molecular modeling of the Arthrobacter sarcosine oxidase.
Nishiya, Y. and Hirayama, N.
J. Anal. Bio-Sci.25 343 - 346 2002
Joint Work
Research paper (scientific journal)
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Homogenization of tissue samples using a split-pestle.
Kusumoto, M., Ikeda, K., Nishiya, Y., and Kawamura, Y.
Anal. Biochem.294 185 - 186 2001
Joint Work
Research paper (scientific journal)
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Characterization and structural prediction of the Arthrobacter creatininase.
Nishiya, Y., Toda, A., and Oka, M.
J. Anal. Bio-Sci.24 144 - 149 2001
Joint Work
Research paper (scientific journal)
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臨床検査用酵素の実用化技術の展開：クレアチニン分解酵素群の開発.
西矢芳昭，山本和巳，川村良久，愛水重典
日本農芸化学会誌75 857 - 862 2001
Joint Work
Research paper (scientific journal)
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Boom法と磁性体粒子を利用したハイスループット核酸精製.
池田勝徳，鈴木正宏，西矢芳昭
生物工学会誌79 22 - 23 2001
Joint Work
Research paper (scientific journal)
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Spontaneous reactivation of Shiga toxins in Escherichia coli O157:H7 cells caused by transposon excision.
Kusumoto, M., Okitsu, T., Nishiya, Y., Suzuki, R., Yamai, S., and Kawamura, Y.
J. Biosci. Bioeng.92 114 - 120 2001
Joint Work
Research paper (scientific journal)
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Identification of Shiga toxin-producing Escherichia coli possessing insertionally inactivated Shiga toxin gene.
Okitsu, T., Kusumoto, M., Suzuki, R., Sata, S., Nishiya, Y., Kawamura, Y., and Yamai, S.
Microbiol. Immunol.45 319 - 322 2001
Joint Work
Research paper (scientific journal)
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Molecular modeling and substrate binding study of the Bacillus stearothermophilus malate dehydrogenase.
Nishiya, Y. and Hirayama, N.
J. Anal. Bio-Sci.23 117 - 122 2000
Joint Work
Research paper (scientific journal)
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The full DNA sequence of the gene encoding the diagnostic enzyme Bacillus uricase.
Nishiya, Y., Hibi, T., and Oda, J.
J. Anal. Bio-Sci.23 443 - 446 2000
Joint Work
Research paper (scientific journal)
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A mutant sarcosine oxidase in which activity depends on flavin adenine dinucleotide.
Nishiya, Y.
Protein Expr. Purif.20 95 - 97 2000
Single Work
Research paper (scientific journal)
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微弱発光撮影装置の機能と応用について.
鈴木正宏，西矢芳昭
遺伝子医学14 148 - 150 2000
Joint Work
Research paper (scientific journal)
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Reactivation of insertionally inactivated Shiga toxin 2 genes of Escherichia coli O157:H7 caused by nonreplicative transposition of the insertion sequence.
Kusumoto, M., Nishiya, Y., and Kawamura, Y.
Appl. Environ. Microbiol.66 1133 - 1138 2000
Joint Work
Research paper (scientific journal)
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Alteration of substrate affinity of Streptomyces cholesterol oxidase for application to the rate assay of cholesterol in serum.
Nishiya, Y. and Hirayama, N.
Clin. Chim. Acta.287 111 - 122 1999
Joint Work
Research paper (scientific journal)
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A model of the polymerase domain in Thermus thermophilus HB8 DNA polymerase.
Nishiya, Y. and Hirayama, N.
J. Anal. Bio-Sci.22 358 - 361 1999
Joint Work
Research paper (scientific journal)
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Identification of an insertion sequence, IS1203 variant, in a Shiga toxin 2 gene of Escherichia coli O157:H7.
Kusumoto, M., Nishiya, Y., Kawamura, Y., and Shinagawa, K.
J. Biosci. Bioeng.87 93 - 96 1999
Joint Work
Research paper (scientific journal)
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Separation of large DNA molecules using a simple field inversion gel electrophoresis.
Nishiya, Y., Yamamoto, Y., and Tsutsui, N.
J. Anal. Bio-Sc.21 289 - 292 1998
Joint Work
Research paper (scientific journal)
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Gene cloning, purification, and characterization of a lactate oxidase from Lactococcus lactis subsp. cremoris IFO3427.
Toda, A. and Nishiya, Y.
J. Ferment. Bioeng.85 507 - 510 1998
Joint Work
Research paper (scientific journal)
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Thermostable glycerol kinase from Thermus flavus: cloning, sequencing, and expression of the enzyme gene.
Huang, H., Kabashima, T., Ito, K., Yin, C-H., Nishiya, Y., Kawamura, Y., and Yoshimoto, T.
Biochim. Biophys. Acta1382 186 - 190 1998
Joint Work
Research paper (scientific journal)
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Effect of non-ionic detergents on apparent enzyme mechanism: V121A mutant of Streptomyces cholesterol oxidase endowed with enhanced sensitivity against detergents.
Nishiya, Y., Yamashita, M., Murooka, Y, Fujii, I., and Hirayama, N.
Protein Engineering11 609 - 611 1998
Joint Work
Research paper (scientific journal)
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Gene cluster of creatinine degradation in Arthrobacter sp. TE1826.
Nishiya, Y., Toda, A., and Imanaka, T.
Mol. Gen. Genet.257 581 - 586 1998
Joint Work
Research paper (scientific journal)
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Improvement of thermal stability of a diagnostic enzyme, Streptomyces cholesterol oxidase, by random and site-directed mutagenesis and a structural interpretation.
Murooka, Y., Nishiya, Y., Toyama, M., Aoike, M., Yamashita, M., and Hirayama, N.
Stability and Stabilization of Biocatalysts, Progress in Biotechnology 15 295 - 302 1998
Joint Work
Research paper (international conference proceedings)
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Purification and characterization of a novel glycine oxidase from Bacillus subtilis.
Nishiya, Y. and Imanaka, T.
FEBS Lett.438 263 - 266 1998
Joint Work
Research paper (scientific journal)
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Improvement of thermal stability of Streptomyces cholesterol oxidase by random mutagenesis and a structural interpretation.
Nishiya, Y., Harada, N., Teshima, S., Yamashita, M., Fujii, I., Hirayama, N., and Murooka, Y.
Protein Engineering10 231 - 235 1997
Joint Work
Research paper (scientific journal)
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Purification and characterization of thermostable glycerol kinase from Thermus flavus.
Huang, H., Yoshida, T., Meng, Y., Kabashima, T., Ito, K., Nishiya, Y., Kawamura, Y., and Yoshimoto, T.
J. Ferment. Bioeng.83 328 - 332 1997
Joint Work
Research paper (scientific journal)
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タンパク質工学的に改変されたFAD(フラビンアデニンジヌクレオチド)含有酸化酵素の諸性質と酵素的測定法への応用.
西矢芳昭，川村良久
生物試料分析20 149 - 156 1997
Joint Work
Research paper (scientific journal)
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Highly conservative sequence in carboxyl terminus of sarcosine oxidase is important for substrate binding.
Nishiya, Y. and Imanaka, T.
J. Ferment. Bioeng.84 591 - 593 1997
Joint Work
Research paper (scientific journal)
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Effects of chloride ion on activity and stability of sarcosine oxidase.
Nishiya, Y. and Kawamura, Y.
J. Anal. Bio-Sci.20 375 - 377 1997
Joint Work
Research paper (scientific journal)
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Enzymatic assay for chloride ion with chloride-dependent sarcosine oxidase created by site-directed mutagenesis.
Nishiya, Y., Kawamura, Y., and Imanaka, T.
Anal. Biochem.245 127 - 132 1997
Joint Work
Research paper (scientific journal)
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Analysis of interaction between the Arthrobacter sarcosine oxidase and the coenzyme flavin adenine dinucleotide by site-directed mutagenesis.
Nishiya, Y. and Imanaka, T.
Appl. Environ. Microbiol.62 2405 - 2410 1996
Joint Work
Research paper (scientific journal)
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Analysis of a negative regulator, SoxR, for the Arthrobacter sarcosine oxidase gene.
Nishiya, Y. and Imanaka, T.
J. Ferment. Bioeng.81 64 - 67 1996
Joint Work
Research paper (scientific journal)
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Investigation of some properties of urea amidolyase from Saccharomyces cerevisiae for application to the enzymatic assays.
Nishiya, Y., Hattori, T., Toda, A., and Kawamura, Y.
J. Anal. Bio-Sci.18 102 - 107 1995
Joint Work
Research paper (scientific journal)
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Active site analysis and stabilization of sarcosine oxidase by the substitution of cysteine residues.
Nishiya, Y., Zuihara, S., and Imanaka, T.
Appl. Environ. Microbiol.61 367 - 370 1995
Joint Work
Research paper (scientific journal)
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組換え酵母によるウレアアミドリアーゼの生産と尿素の酵素的測定への利用.
西矢芳昭，戸田篤志，川村良久
生物試料分析18 288 - 293 1995
Joint Work
Research paper (scientific journal)
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Primary structure of the ADE1 gene from Candida utilis.
Nishiya, Y.
Biosci. Biotech. Biochem.58 208 - 210 1994
Single Work
Research paper (scientific journal)
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Alteration of substrate specificity and optimum pH of sarcosine oxidase by random and site-directed mutagenesis.
Nishiya, Y. and Imanaka, T.
Appl. Environ. Microbiol.60 4213 - 4215 1994
Joint Work
Research paper (scientific journal)
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Cloning and nucleotide sequence of the urea amidolyase gene from Candida utilis.
Nishiya, Y. and Imanaka, T.
J. Ferment. Bioeng.75 245 - 253 1993
Joint Work
Research paper (scientific journal)
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Cloning and sequencing of the sarcosine oxidase gene from Arthrobacter sp. TE1826.
Nishiya, Y. and Imanaka, T.
J. Ferment. Bioeng.75 239 - 244 1993
Joint Work
Research paper (scientific journal)
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Cloning and nucleotide sequences of the Bacillus stearothermophilus neutral protease gene and its transcriptional activator gene.
Nishiya, Y. and Imanaka, T.
J. Bacteriol.172 4861 - 4869 1990
Joint Work
Research paper (scientific journal)