Papers - OYAMA Hiroshi
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Characterization of proteinous coagulant in Moringa tree seeds for water purification: Stepwise laboratory exercise for high-school students
Hiroshi Oyama, Yuka Nabeshima, Koichi Morimoto, Yukio Sugimura
Biochemistry and Molecular Biology Education51 ( 5 ) 588 - 599 2023.06
Joint Work
Research paper (scientific journal)
Authorship: Lead author Corresponding author
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Site-Selective Incorporation of a Functional Group into Lys175 in the Vicinity of the Active Site of Chymotrypsin by Using Peptidyl α-Aminoalkylphosphonate Diphenyl Ester-Derivatives.
S. Ono, M. Koga, Y. Arimura, T. Hatakeyama, M. Kobayashi, J. Sagara, T. Nakai, Y. Horino, H. Kuroda, H. Oyama, K. Arima
Molecules28 ( 7 ) 3150 2023.03
Single Work
Research paper (scientific journal)
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Analysis of functional ingredients of tempe-like fermented Moringa oleifera seeds (Moringa tempe) prepared with Rhizopus species
Hideyuki Aoki, Tomoko Nakatsuka-Mori,Yoshie Ueno, Yuka Nabeshima, Hiroshi Oyama
Journal of Bioscience and Bioengineering135 ( 4 ) 306 - 312 2023.02
Joint Work
Research paper (scientific journal)
Authorship: Last author Corresponding author
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Fabrication of high-power density biofuel cell using sericin electrolyte and anomalous proton conductivity
Takashi Kawabata, Yasumitsu Matsuo, Hidetoshi Teramoto, Hiroshi Oyama
Sustainable Materials and Technologies32 e00440 2022.07
Joint Work
Research paper (scientific journal)
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Comparison of Bioactive Components in Tempeh Produced by Three Different Rhizopus Starters and Immunomodulatory Effect of Tempeh on Atopic Dermatitis Mice
Hideyuki Aoki, Shunsuke Chuma, Yoshinori Iba, Haruka Tashiro, Nakamichi Watanabe, Hiroshi Oyama
Food Science and Technology Research26 ( 5 ) 665 - 672 2020.09
Joint Work
Research paper (scientific journal)
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Experimental teaching materials to learn germination process of rice seeds. Ⅰ. Induction of amylase synthesis by gibberellin.
Oyama, H., Morimoto, K. and Sugimura, Y
生物教育61 ( 1 ) 31 - 40 2019.12
Joint Work
Research paper (scientific journal)
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Bioluminescence Microplate Assay of Cyanide with Escherichia coli Harboring a Plasmid Responsible for Cyanide-dependent Light Emission in Alginate Microenvironment
Hajime KARATANI, Yasuro FUSE, Hirotaka MIZUGUCHI, Shogo MONJI, Hiroshi OYAMA, Tomonori WAKU, Masashi IWASAKI
Analytical Sciences35 ( 7 ) 821 - 825 2019.07
Joint Work
Research paper (scientific journal)
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Correction to “Inhibitor Complexes of the Pseudomonas Serine-Carboxyl Proteinase”
Alexander Wlodawer, Mi Li, Alla Gustchina, Zbigniew Dauter, Kenichi Uchida, Hiroshi Oyama, Nathan E. Goldfarb, Ben M. Dunn, Kohei Oda
Biochemistry57 ( 4 ) 6644 - 6644 2018.11
Joint Work
Research paper (scientific journal)
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Cloning, Purification, and Characterization of Tripeptidyl Peptidase from Streptomyces herbaricolor TY-21
Ekino K., Yonei S., Oyama H., Oka T., Nomura Y., Shin T.
Applied Biochemistry and Biotechnology184 ( 1 ) 239 - 252 2018.01
Joint Work
Research paper (scientific journal)
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Innate immune responses in the silkworm, Bombyx mori-Phagocytosis of hemocytes and inducible synthesis of antibacterial proteins-
Sugimura, Y., Morimoto, K. and Oyama, H.
Japanese Journal of Biological Education59 ( 1 ) 10 - 18 2017.12
Joint Work
Research paper (scientific journal)
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Oral Administration of Collagen Hydrolysates Improves Glucose Tolerance in Normal Mice Through GLP-1-Dependent and GLP-1-Independent Mechanisms.
Iba Y., Yokoi K., Eitoku I., Goto M., Koizumi S., Sugihara F., Oyama H., Yoshimoto T.
Journal of Medicinal Food 19 ( 9 ) 836 - 843 2016.09
Joint Work
Research paper (scientific journal)
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Site-Selective Chemical Modification of Chymotrypsin Using Peptidyl Derivatives Bearing Optically Active Diphenyl 1-Amino-2-Phenylethylphosphonate: Stereochemical Effect of the Diphenyl Phosphonate Moiety.
Shin Ono, et al.
Peptide Science 2015.12
Joint Work
Research paper (scientific journal)
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Sex determination of the silkworm, Bombyx mori,by manual polymerase chain reaction.
Sugimura, Y., Oyama, H.and Morimoto, K.
JapaneseJ. Biological Education56 ( 1 ) 29 - 35 2015.12
Joint Work
Research paper (scientific journal)
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カイコ幼虫の消化液に含まれるアミラーゼとプロテアーゼを用いた新しい教材開発
森本弘一、尾山廣、杉村順夫
生物教育55 ( 1 ) 2月13日 2014.12
Joint Work
Research paper (scientific journal)
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アミラーゼおよびプロテアーゼ活性の半定量的簡易検出法の開発と応用
森本弘一、尾山廣、杉村順夫
生物教育54 ( 2 ) 60 - 67 2014.05
Joint Work
Research paper (scientific journal)
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Covalent chromatography for chymotrypsin-like proteases using a diphenyl 1-amino-2-phenylethylphosphonate derivative
Shin Ono, Junya Murai, Seigo Furuta, et al.
Journal of Biological Macromolecules13 78 - 85 2013.12
Joint Work
Research paper (scientific journal)
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Site-selective Chemical Modification of Chymotrypsin Using a Peptidyl Diphenyl 1-Amino-2-phenylethylphosphonate Derivative
Shin Ono, Junya Murai, Takahiko Nakai, et al.
Chemistry Letters42 ( 8 ) 860 - 862 2013.08
Joint Work
Research paper (scientific journal)
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Assessment of substrate inhibition of bacterial oligopeptidase B
Mohamed Mustafa MS, Nakajima Y, Oyama H,
Biological and Pharmaceutical Bulletin35 ( 11 ) 2010 - 2016 2012.11
Joint Work
Research paper (scientific journal)
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Proton Conduction and Impedance Analysis In Submucosa Membrane
YASUMITSU MATSUO, JUNKO HATORI, HIROSHI OYAMA
Solid State Ionics （Ionics for Sustainable World） 333 - 339 2012.10
Joint Work
Research paper (scientific journal)
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Substitution of Glu122 by glutamine revealed the function of the second water molecule as a proton donor in the binuclear metal enzyme creatininase.
Yamashita, et al.
Journal of Molecular Biology394 ( 4 ) 1081 - 1096 2010.03
Joint Work
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Dipeptidyl aminopeptidase IV from Stenotrophomonas maltophilia exhibits activity against a substrate containing a 4-hydroxyproline residue.
中嶋義隆、伊藤潔、十島翼、永川貴、Ｚｈｅｎｇ，Ｈ．、Ｗｕ，ＹＦ．、高橋栄二、京野潔、芳本忠
Ｊｏｕｒｎａｌ ｏｆ Ｂａｃｔｅｒｉｏｌｏｇｙ190 ( 23 ) 7819 - 7829 2008.09
Joint Work
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Novel inhibitor for prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis and details of substrate-recognition mechanism.
Ｘｕ，Ｙ．、中嶋義隆、伊藤潔、Ｚｈｅｎｇ，Ｈ．、Ｈｅｉｓｅｒ，Ｕ．、Ｈｏｆｆｍａｎｎ，Ｔ．、Ｇａｒｔｎｅｒ，ＵＴ．、Ｄｅｍｕｔｈ，ＨＵ．、芳本忠
Ｊｏｕｒｎａｌ ｏｆ Ｍｏｌｅｃｕｌａｒ Ｂｉｏｌｏｇｙ375 ( 3 ) 708 - 719 2008.01
Joint Work
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Oligonucleotide transformation for the study of mutagenic specificities of DNA lesions in yeast.
根岸和雄、関根大二郎、森光巧、鈴木哲夫、奥川洋司、川上朝子、大塚智恵、Ｌｏａｋｅｓ，Ｄ．
Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｓｙｍｐ Ｓｅｒ （Ｏｘｆ）51 ( 1 ) 211 - 212 2007.11
Joint Work
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Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site.
大久保安由美、Ｌｉ，Ｍ．、芦田理子、Ｇｕｓｔｃｈｉｎａ，Ａ．、小田耕平、Ｄｕｎｎ，ＢＭ．、Ｗｌｏｄａｗｅｒ，Ａ．、中山亨
ＦＥＢＳ Ｊ．273 ( 11 ) 2563 - 2576 2006.06
Joint Work
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Synergetic effects of pressure and chemical denaturant on protein unfolding: stability of a serine-type carboxyl protease, kumamolisin.
藤本泰範、池内秀和、多田朋子、小田耕平、功刀滋
Ｂｉｏｃｈｉｍ．Ｂｉｏｐｈｙｓ．Ａｃｔａ1764 ( 3 ) 364 - 371 2006.03
Joint Work
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Molecular cloning of the gene encoding Vibrio metalloproteinase vimelysin and isolation of a mutant with high stability in organic solvents.
高橋敏宏、Ｎｇ，ＫＫ．、小田耕平
Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｃｈｅｍｉｓｔｒｙ （Ｔｏｋｙｏ）138 ( 6 ) 701 - 710 2005.12
Joint Work
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Purification and characterisation of keratinase from a thermotolerant feather-degrading bacterium.
Suntornsuk,W.,Tongjun,J.,Onnim,P.,Ratanakanokchai,K.,Kusamran,T.,小田耕平
Ｗｏｒｌｄ Ｊ．Ｍｉｃｒｏｂｉｏｌ．Ｂｉｏｔｅｃｈ．21 ( 40701 ) 1111 - 1117 2005.10
Joint Work
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Catalytic residues and substrate specificity of recombinant human tripeptidyl peptidase I (CLN2).
藤澤智子、鈴木健夫、Ｗｌｏｄａｗｅｒ，Ａ．、Ｄｕｎｎ，ＢＭ．、小田耕平
Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｃｈｅｍｉｓｔｒｙ （Ｔｏｋｙｏ）138 ( 2 ) 127 - 134 2005.08
Joint Work
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Catalytic residues and substrate specificity of scytalidoglutamic peptidase, the first member of theeqolisin in family (G1) of peptidases.
片岡由起子、高田克巳、常見雅彦、Ｊａｍｅｓ，ＭＮ．、小田耕平
ＦＥＢＳ Ｌｅｔｔ．579 ( 14 ) 3364 - 3369 2005.06
Joint Work
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Crystallographic and biochemical investigations of kumamolisin-As, a serine-carboxyl peptidase with collagenase activity.
Wlodawer,A.,Li,M.,Gustchina,A.,鶴岡直樹,芦田理子,南方宏之,小田耕平,西野徳三,中山亨
Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．279 ( 20 ) 21500 - 21510 2004.10
Joint Work
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1.2 A crystal structure of the serine carboxyl proteinase pro-kumamolisin: structure of an intact pro-subtilase.
Comellas-Bigler,M.,Maskos,K.,Huber,R.,小田耕平,Bode,W.
Ｓｔｒｕｃｔｕｒｅ（Ｃａｍｂ）12 ( 7 ) 1313 - 1323 2004.07
Joint Work
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Two inhibitor molecules bound in the active site of Pseudomonas sedolisin: a model for the bi-product complex following cleavage of a peptide substrate.
Wlodawer,A.,Li,M.,Gustchina,A.,小田耕平,Beyer,BB.,Clemente,J.,Dunn,BM.
Ｂｉｏｃｈｅｍ．Ｂｉｏｐｈｙｓ Ｒｅｓ．Ｃｏｍｍｕｎ．314 ( 2 ) 3364 - 3369 2004.06
Joint Work
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The molecular structure and catalytic mechanism of a novel carboxyl peptidase from Scytalidium lignicolum.
Fujinaga,M.,Cherney,MM.,小田耕平,James,MN.
Ｐｒｏｃ．Ｎａｔｌ．Ａｃａｄ．Ｓｃｉ．ＵＳＡ．101 ( 10 ) 3364 - 3369 2004.05
Joint Work
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Purification and characterization of alkaline serine proteinase from photosynthetic bacterium,Rubrivivax gelatinosus KDDS1
小田耕平、Ｔａｎｓｋｕｌ，Ｓ．、Ｎｏｐａｒａｔｎａｒａｐｏｒｎ，Ｎ．
Ｂｉｏｓｃｉｅｎｃｅ，Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ68 ( 3 ) 650 - 655 2004.03
Joint Work
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Substrate specificity of alkaline serine proteinase isolated from photosynthetic bacterium,Rubrivivax gelatinosus KDDS1.
Tanskul,S.,小田耕平,Noparatnaraporn,N.,常見雅彦,高田克巳
Ｂｉｏｃｈｅｍ．Ｂｉｏｐｈｙｓ Ｒｅｓ．Ｃｏｍｍｕｎ．309 ( 3 ) 547 - 551 2003.03
Joint Work
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A model of tripeptidyl-peptidase I(CLN2), a ubiquitous and highly conserved member of the sedolisin family of serine-carboxyl peptidases.
Wlodawer,A.,Durell,SR.,Li,M.,小田耕平,Dunn,BM.
ＢＭＣ Ｓｔｒｕｃｔ．Ｂｉｏｌ． ( 40610 ) 2003.03
Joint Work
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Collagenolytic serine-carboxyl proteinase from Alicyclobacillus sendaiensis strain NTAP-1:purification,characterization,gene cloning,and heterologous expression.
鶴岡直樹、中山亨、芦田理子、邊見久、中尾正宏、南方宏之、小田耕平、西野徳三
Ａｐｐｌｉｅｄ ａｎｄ Ｅｎｖｉｒｏｎｍｅｎｔａｌ Ｍｉｃｒｏｂｉｏｌｏｇｙ69 ( 1 ) 162 - 169 2003.01
Joint Work
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The 1.4 A crystal structure of kumamolysin:A thermostable serine-carboxy-type proteinase.
Comellas-Bigler,M.,Fuentes-Prior,P.,Maskos,K.,Huber,R.,内田健一,Dunn,BM.,小田耕平,Bode,W.
Ｓｔｒｕｃｔｕｒｅ（Ｃａｍｂ）10 865 - 876 2002.06
Joint Work
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A CLN2-related and thermostable serine-carboxyl proteinase,kumamolysin:cloning,expression,and identification of catalytic serine residue.
浜田貴俊、小笠原心、内田健一、村尾澤夫、Ｂｅｙｅｒ，ＢＢ．、Ｄｕｎｎ，ＢＭ．、小田耕平
Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｃｈｅｍｉｓｔｒｙ（Ｔｏｋｙｏ）131 ( 5 ) 757 - 765 2002.05
Joint Work
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Inhibitor complexes of the Pseudomonas serine-carboxyl proteinase.
Wlodawer,A.,Li,M.,Gustchina,A.,Dauter,Z.,内田健一,Goldfarb,NE.,Dunn,BM.,小田耕平
Ｂｉｏｃｈｅｍｉｓｔｒｙ40 ( 51 ) 15602 - 15611 2001.12
Joint Work
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Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes.
Wlodawer,A.,Li,M.,Dauter,Z.,Gustchina,A.,内田健一,Dunn,BM.,小田耕平
Ｎａｔｕｒｅ Ｓｔｒｕｃｔｕｒａｌ Ｂｉｏｌｏｇｙ8 ( 5 ) 442 - 446 2001.05
Joint Work
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A simultaneous assay method for L-glutamate and L-pyroglutamate contents in soy sauce using a 5-oxoprolinase (without ATP hydrolyzing activity).
西村篤寿、伊藤誉、村尾澤夫、小田耕平
Ｂｉｏｓｃｉｅｎｃｅ，Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ65 ( 2 ) 477 - 479 2001.02
Joint Work
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Effects of pressure on the activity and spectroscopic properties of carboxyl proteinases. Apparent correlation of pepstatin-insensitivity and pressure response.
藤原聡、功刀滋、小田耕平
Ｅｕｒｏｐｅａｎ Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｃｈｅｍｉｓｔｒｙ268 645 - 655 2001.02
Joint Work
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Cloning and rational mutagenesis of kextatin I,a potent proteinaceous inhibitor of kex 2 proteinase
小田耕平、伊東里志、吹春桃子、宮川優、高橋砂織、広瀬未果、菊池典久、中山亨、柴野祐次
Ｂｉｏｃｈｅｍｉｃａｌ Ｊｏｕｒｎａｌ 355 339 - 346 2001.02
Joint Work
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Molelcular cloning,sequencing,and expressionin Escherichia coli of the gene encoding a novel 5- oxoprolinase without ATP-hydrolyzing activity from Alcaligenes faecalis N-38A.
西村篤寿、浜田貴俊、信岡克典、新隆志、村尾澤夫、小田耕平
Ａｐｐｌｉｅｄ ａｎｄ Ｅｎｖｉｒｏｎｍｅｎｔａｌ Ｍｉｃｒｏｂｉｏｌｏｇｙ66 ( 8 ) 3201 - 3205 2000.08
Joint Work
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Expression and secretion of Scytalidopepsin B,an acid protease from Scytalidium lignicolum,in yeast.
志牟田健、小田－上田直子、鷲尾正博、小田耕平、鶴大典
Ｂｉｏｓｃｉｅｎｃｅ，Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ64 ( 7 ) 1542 - 1546 2000.07
Joint Work
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Subsite preferences of pepstatin-insensitive carboxyl proteinases from prokaryotes: Kumamolysin,a thermostable pepstatin-insensitive carboxyl proteinase.
小田耕平、小笠原心、Ｄｕｎｎ，Ｂ．Ｍ．
Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｃｈｅｍｉｓｔｒｙ （Ｔｏｋｙｏ）128 ( 3 ) 499 - 507 2000.03
Joint Work
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Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesis.
安部慎一郎、牛山聡子、高橋砂織、小田耕平
Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ274 ( 39 ) 27815 - 27822 1999.09
Joint Work
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Purification and Characterization of a novel 5-oxoprolinease (Without ATP-hydrolyzing activity) from Alcaligenes faecalis N-38A.
西村篤寿、尾崎安宣、新隆志、村尾澤夫
Ａｐｐｌｉｅｄ Ｅｎｖｉｒｏｎｍｅｎｔａｌ Ｍｉｃｒｏｂｉｏｌｏｇｙ 65 ( 2 ) 712 - 717 1999.02
Joint Work
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Purification and Characterization of a Prolyl Endopeptidase from Pseudomonas sp.KU-22.
青木秀之、天野誠、芳本忠、鶴大典、新隆志、村尾澤夫
Ｊｏｕｒｎａｌ ｏｆ Ｆｅｒｍｅｎｔａｔｉｏｎ ａｎｄ Ｂｉｏｅｎｇｉｎｅｅｒｉｎｇ84 ( 6 ) 538 - 542 1997.06
Joint Work
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Purification and Characterization of an Alkaline Proteinase Produced by Pimelobacter sp.Z-483.
金城充、渡満則、新隆志、村尾澤夫
Ｊｏｕｒｎａｌ ｏｆ Ｆｅｒｍｅｎｔａｔｉｏｎ ａｎｄ Ｂｉｏｅｎｇｉｎｅｅｒｉｎｇ84 ( 4 ) 351 - 353 1997.04
Joint Work
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A Novel Type of Proteinase,Masikinolysin,from Streptomyces griseoloalbus SN-22.
村尾澤夫、藤野博昭、新隆志
Ｂｉｏｓｃｉｅｎｃｅ，Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ58 ( 12 ) 2308 - 2309 1994.12
Joint Work
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Purification and Characterization of Kumamolysin,a Novel Thermostable Pepstatin-insensitive Carboxyl Proteinase from Bacillus novosp.MN-32.
村尾澤夫、大国啓造、長尾正憲、平山和雄、福原健一、小田耕平、新隆志
Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ268 ( 1 ) 349 - 355 1993.01
Joint Work
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Cloning and Expression of Subtilisin Amylosacchariticus Gene.
芳本忠、本多隆、刀禰寛、竹下智章、神山朝光、鶴大典
Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｃｈｅｍｉｓｔｒｙ （Ｔｏｋｙｏ）103 ( 6 ) 1060 - 1065 1988.06
Joint Work